© 2014 American Institute of Chemical Engineers. (Capto Adhere- 492, Capto Q- 477 cm/hr), whilst Sepharose CL-6B, Sepharose 4. Latin hypercube sampling SMA model arginine hydrochloride calcium chloride multimodal chromatography. The results showed that the Capto family had the highest critical velocities. The results in this article establish the utility of multimodal chromatography when used with appropriate mobile phase modifiers for the downstream bioprocessing of a modified human growth hormone and offer new approaches for bioprocess verification. Finally, justification of the selected operating ranges is evaluated using the model together with Latin hypercube sampling. Capto adhere is a multimodal ion exchanger and is designed to be used as a second or third step in monoclonal antibody (MAb) purification processes, (i.e., after capture on a Protein A medium). Furthermore, the steric mass action model is shown to be capable of accurately describing the chromatographic process and the aggregate removal. Capto adhere is a multimodal strong anion exchanger for BioProcess applications. Importantly, these processes are shown to result in significant clearance of product related aggregates and host cell proteins. This study demonstrates how the multimodal Capto adhere resin can be used in concert with calcium chloride or arginine hydrochloride as mobile phase modifiers to create a highly selective purification process for a modified human growth hormone. HiTrap Capto adhere is prepacked with Capto adhere, a multimodal BioProcess ion exchange resin designed to remove contaminats in post-protein A purification.Chromatography columns separate molecules within mixtures during gas or liquid chromatography.
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